A compact fiber-optic probe-based singlet oxygen luminescence detection system

Nathan R. Gemmell, Aongus McCarthy, Michele M. Kim, Israel Veilleux, Timothy C. Zhu, Gerald Stuart Buller, Brian C. Wilson, Robert H. Hadfield

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16 Citations (Scopus)
35 Downloads (Pure)

Abstract

This paper presents a novel compact fiberoptic based singlet oxygen near-infrared luminescence probe coupled to an InGaAs/InP single photon avalanche diode (SPAD) detector. Patterned time gating of the single-photon detector is used to limit unwanted dark counts and eliminate the strong photosensitizer luminescence background. Singlet oxygen luminescence detection at 1270 nm is confirmed through spectral filtering and lifetime fitting for Rose Bengal in water, and Photofrin in methanol as model photosensitizers. The overall performance, measured by the signal-to-noise ratio, improves by a factor of 50 over a previous system that used a fiberoptic-coupled superconducting nanowire single-photon detector. The effect of adding light scattering to the photosensitizer is also examined as a first step towards applications in tissue in vivo.

Original languageEnglish
Pages (from-to)320-326
JournalJournal of Biophotonics
Volume10
Issue number2
Early online date25 Jul 2016
DOIs
Publication statusPublished - 3 Feb 2017

Keywords

  • Fluorescence
  • Photon counting
  • Quantum optics
  • Singlet oxygen

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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  • Cite this

    Gemmell, N. R., McCarthy, A., Kim, M. M., Veilleux, I., Zhu, T. C., Buller, G. S., Wilson, B. C., & Hadfield, R. H. (2017). A compact fiber-optic probe-based singlet oxygen luminescence detection system. Journal of Biophotonics, 10(2), 320-326. https://doi.org/10.1002/jbio.201600078