A Bacillus thuringiensis strain producing a polyglutamate capsule resembling that of Bacillus anthracis

Elise Cachat; Margaret Barker; Timothy D. Read; Fergus G. Priest

doi:10.1111/j.1574-6968.2008.01231.x

A Bacillus thuringiensis strain producing a polyglutamate capsule resembling that of Bacillus anthracis

Elise Cachat, Margaret Barker, Timothy D. Read, Fergus G. Priest

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Bacillus thuringiensis serovar Monterrey strain BGSC 4AJ1 produced a microscopically visible capsule that reacted with a fluorescent antibody specific for the poly-?-d-glutamic acid (PGA) capsule of Bacillus anthracis. PGA capsule biosynthesis genes with 75%, 81%, 72%, 65% and 63% similarity, respectively, to those of the B. anthracis capBCADE cluster were present on a plasmid (pAJ1-1). Strain BGSC 4AJ1, together with five strains of Bacillus cereus that hybridized to a PGA cap gene probe, were analyzed phylogenetically using six housekeeping genes of a B. cereus multilocus sequence typing scheme. Bacillus thuringiensis BGSC 4AJ1 shared four identical alleles with B. anthracis and was the second most closely related to this bacterium of the 674 isolates in the multilocus sequence typing database. The other cap ⁺ strains were distributed among various lineages of Clade 1 of the B. cereus group. © 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

Original language	English
Pages (from-to)	220-226
Number of pages	7
Journal	FEMS Microbiology Letters
Volume	285
Issue number	2
DOIs	https://doi.org/10.1111/j.1574-6968.2008.01231.x
Publication status	Published - Aug 2008

Keywords

Bacillus anthracis
Bacillus cereus
Bacillus thuringiensis
Capsule
MLST
Polyglutamic acid

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10.1111/j.1574-6968.2008.01231.x

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title = "A Bacillus thuringiensis strain producing a polyglutamate capsule resembling that of Bacillus anthracis",

abstract = "Bacillus thuringiensis serovar Monterrey strain BGSC 4AJ1 produced a microscopically visible capsule that reacted with a fluorescent antibody specific for the poly-?-d-glutamic acid (PGA) capsule of Bacillus anthracis. PGA capsule biosynthesis genes with 75%, 81%, 72%, 65% and 63% similarity, respectively, to those of the B. anthracis capBCADE cluster were present on a plasmid (pAJ1-1). Strain BGSC 4AJ1, together with five strains of Bacillus cereus that hybridized to a PGA cap gene probe, were analyzed phylogenetically using six housekeeping genes of a B. cereus multilocus sequence typing scheme. Bacillus thuringiensis BGSC 4AJ1 shared four identical alleles with B. anthracis and was the second most closely related to this bacterium of the 674 isolates in the multilocus sequence typing database. The other cap + strains were distributed among various lineages of Clade 1 of the B. cereus group. {\textcopyright} 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.",

keywords = "Bacillus anthracis, Bacillus cereus, Bacillus thuringiensis, Capsule, MLST, Polyglutamic acid",

author = "Elise Cachat and Margaret Barker and Read, {Timothy D.} and Priest, {Fergus G.}",

year = "2008",

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doi = "10.1111/j.1574-6968.2008.01231.x",

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N2 - Bacillus thuringiensis serovar Monterrey strain BGSC 4AJ1 produced a microscopically visible capsule that reacted with a fluorescent antibody specific for the poly-?-d-glutamic acid (PGA) capsule of Bacillus anthracis. PGA capsule biosynthesis genes with 75%, 81%, 72%, 65% and 63% similarity, respectively, to those of the B. anthracis capBCADE cluster were present on a plasmid (pAJ1-1). Strain BGSC 4AJ1, together with five strains of Bacillus cereus that hybridized to a PGA cap gene probe, were analyzed phylogenetically using six housekeeping genes of a B. cereus multilocus sequence typing scheme. Bacillus thuringiensis BGSC 4AJ1 shared four identical alleles with B. anthracis and was the second most closely related to this bacterium of the 674 isolates in the multilocus sequence typing database. The other cap + strains were distributed among various lineages of Clade 1 of the B. cereus group. © 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

AB - Bacillus thuringiensis serovar Monterrey strain BGSC 4AJ1 produced a microscopically visible capsule that reacted with a fluorescent antibody specific for the poly-?-d-glutamic acid (PGA) capsule of Bacillus anthracis. PGA capsule biosynthesis genes with 75%, 81%, 72%, 65% and 63% similarity, respectively, to those of the B. anthracis capBCADE cluster were present on a plasmid (pAJ1-1). Strain BGSC 4AJ1, together with five strains of Bacillus cereus that hybridized to a PGA cap gene probe, were analyzed phylogenetically using six housekeeping genes of a B. cereus multilocus sequence typing scheme. Bacillus thuringiensis BGSC 4AJ1 shared four identical alleles with B. anthracis and was the second most closely related to this bacterium of the 674 isolates in the multilocus sequence typing database. The other cap + strains were distributed among various lineages of Clade 1 of the B. cereus group. © 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

KW - Bacillus anthracis

KW - Bacillus cereus

KW - Bacillus thuringiensis

KW - Capsule

KW - MLST

KW - Polyglutamic acid

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ER -

A Bacillus thuringiensis strain producing a polyglutamate capsule resembling that of Bacillus anthracis

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Keywords

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