A 3D mammalian cell separator biochip

Debaditya Choudhury, William T. Ramsay, Robert Kiss, Nicholas A. Willoughby, Lynn Paterson, Ajoy K. Kar

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)
114 Downloads (Pure)


The dissimilar cytoskeletal architecture in diverse cell types induces a difference in their deformability that presents a viable approach to separate cells in a non-invasive manner. We report on the design and fabrication of a robust and scalable device capable of separating a heterogeneous population of cells with variable degree of deformability into enriched populations with deformability above a certain threshold. The three dimensional device was fabricated in fused silica by femtosecond laser direct writing combined with selective chemical etching. The separator device was evaluated using promyelocytic HL60 cells. Using flow rates as large as 167 mu L min(-1), throughputs of up to 2800 cells min(-1) were achieved at the device output. A fluorescence-activated cell sorting (FACS) viability analysis on the cells revealed 81% of the population maintain cellular integrity after passage through the device.

Original languageEnglish
Pages (from-to)948-953
Number of pages6
JournalLab on a Chip
Issue number5
Publication statusPublished - 2012


Dive into the research topics of 'A 3D mammalian cell separator biochip'. Together they form a unique fingerprint.

Cite this