A 3D mammalian cell separator biochip

Debaditya Choudhury, William T. Ramsay, Robert Kiss, Nicholas A. Willoughby, Lynn Paterson, Ajoy K. Kar

Research output: Contribution to journalArticle

93 Downloads (Pure)

Abstract

The dissimilar cytoskeletal architecture in diverse cell types induces a difference in their deformability that presents a viable approach to separate cells in a non-invasive manner. We report on the design and fabrication of a robust and scalable device capable of separating a heterogeneous population of cells with variable degree of deformability into enriched populations with deformability above a certain threshold. The three dimensional device was fabricated in fused silica by femtosecond laser direct writing combined with selective chemical etching. The separator device was evaluated using promyelocytic HL60 cells. Using flow rates as large as 167 mu L min(-1), throughputs of up to 2800 cells min(-1) were achieved at the device output. A fluorescence-activated cell sorting (FACS) viability analysis on the cells revealed 81% of the population maintain cellular integrity after passage through the device.

Original languageEnglish
Pages (from-to)948-953
Number of pages6
JournalLab on a Chip
Volume12
Issue number5
DOIs
Publication statusPublished - 2012

Cite this