3D mapping of microfluidic flow in laboratory-on-a-chip structures using optical tweezers

Hasan Mushfique, Jonathan Leach, Huabing Yin, Roberto Di Leonardo, Miles J. Padgett, Jonathan M. Cooper

Research output: Contribution to journalArticle

18 Citations (Scopus)


Accurate measurement of flow in microfluidic systems is both challenging and important, providing information that can be used to better understand flow fields within laboratory-on-a-chip devices and validate computational simulations. Here, we use optical tweezers within a microfluidic system to measure the velocity vectors of flow fields in two and three dimensions around a microstructures including both molded features within channels and cells. The experimental results are compared to a complex fluid dynamics model showing an agreement between the two of better than 3 mu m/s. This measurement is highly reproducible and minimally invasive, which in the future could be used to provided more in-depth studies of the rheological properties of biological cells and microstructures in laboratory-on-a-chip devices.

Original languageEnglish
Pages (from-to)4237-4240
Number of pages4
JournalAnalytical Chemistry
Issue number11
Publication statusPublished - 1 Jun 2008

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