3D Bioprinting of Complex, Cell-laden Alginate Constructs

Atabak Ghanizadeh Tabriz, Dirk-Jan Cornelissen, Wenmiao Shu

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)


Biofabrication has been receiving a great deal of attention in tissue engineering and regenerative medicine either by manual or automated processes. Different automated biofabrication techniques have been used to produce cell-laden alginate hydrogel structures, especially bioprinting approaches. These approaches have been limited to 2D or simple 3D structures, however. In this chapter, a novel bioprinting technique is disclosed for the production of more complex alginate hydrogel structures. This was achieved by dividing the alginate hydrogel cross-linking process into three stages: primary calcium ion cross-linking for printability of the gel, secondary calcium ion cross-linking for rigidity of the alginate hydrogel immediately after printing, and tertiary barium ion cross-linking for the long-term stability of the alginate hydrogel in the culture medium.

Original languageEnglish
Title of host publicationComputer-Aided Tissue Engineering
Place of PublicationNew York
PublisherHumana Press
Number of pages6
ISBN (Electronic)9781071606117
ISBN (Print)9781071606100
Publication statusPublished - 2021

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • 3D bioprinting
  • Alginate hydrogel
  • Bioextrusion
  • Biofabrication

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Tabriz, A. G., Cornelissen, D-J., & Shu, W. (2021). 3D Bioprinting of Complex, Cell-laden Alginate Constructs. In Computer-Aided Tissue Engineering (pp. 143-148). (Methods in Molecular Biology). Humana Press. https://doi.org/10.1007/978-1-0716-0611-7_12