25th Anniversary Article

Engineering Hydrogels for Biofabrication

Jos Malda, Jetze Visser, Ferry P. Melchels, Tomasz Juengst, Wim E. Hennink, Wouter J. A. Dhert, Juergen Groll, Dietmar W. Hutmacher

Research output: Contribution to journalLiterature review

Abstract

With advances in tissue engineering, the possibility of regenerating injured tissue or failing organs has become a realistic prospect for the first time in medical history. Tissue engineering - the combination of bioactive materials with cells to generate engineered constructs that functionally replace lost and/or damaged tissue - is a major strategy to achieve this goal. One facet of tissue engineering is biofabrication, where three-dimensional tissue-like structures composed of biomaterials and cells in a single manufacturing procedure are generated. Cell-laden hydrogels are commonly used in biofabrication and are termed bioinks. Hydrogels are particularly attractive for biofabrication as they recapitulate several features of the natural extracellular matrix and allow cell encapsulation in a highly hydrated mechanically supportive three-dimensional environment. Additionally, they allow for efficient and homogeneous cell seeding, can provide biologically-relevant chemical and physical signals, and can be formed in various shapes and biomechanical characteristics. However, despite the progress made in modifying hydrogels for enhanced bioactivation, cell survival and tissue formation, little attention has so far been paid to optimize hydrogels for the physico-chemical demands of the biofabrication process. The resulting lack of hydrogel bioinks have been identified as one major hurdle for a more rapid progress of the field. In this review we summarize and focus on the deposition process, the parameters and demands of hydrogels in biofabrication, with special attention to robotic dispensing as an approach that generates constructs of clinically relevant dimensions. We aim to highlight this current lack of effectual hydrogels within biofabrication and initiate new ideas and developments in the design and tailoring of hydrogels. The successful development of a printable hydrogel that supports cell adhesion, migration, and differentiation will significantly advance this exciting and promising approach for tissue engineering.

Original languageEnglish
Pages (from-to)5011-5028
Number of pages18
JournalAdvanced Materials
Volume25
Issue number36
DOIs
Publication statusPublished - 25 Sep 2013

Keywords

  • bioprinting
  • biofabrication
  • additive manufacturing
  • hydrogel
  • biomaterials
  • biopolymers
  • DOUBLE-NETWORK HYDROGELS
  • 3-DIMENSIONAL FREEFORM FABRICATION
  • CELL-LADEN
  • ENDOTHELIAL-CELLS
  • CARTILAGE REPAIR
  • CROSS-LINKING
  • BIOMEDICAL APPLICATIONS
  • EXTRACELLULAR MATRICES
  • PRINTING APPLICATIONS
  • TRIBLOCK COPOLYMERS

Cite this

Malda, J., Visser, J., Melchels, F. P., Juengst, T., Hennink, W. E., Dhert, W. J. A., ... Hutmacher, D. W. (2013). 25th Anniversary Article: Engineering Hydrogels for Biofabrication. Advanced Materials, 25(36), 5011-5028. https://doi.org/10.1002/adma.201302042
Malda, Jos ; Visser, Jetze ; Melchels, Ferry P. ; Juengst, Tomasz ; Hennink, Wim E. ; Dhert, Wouter J. A. ; Groll, Juergen ; Hutmacher, Dietmar W. / 25th Anniversary Article : Engineering Hydrogels for Biofabrication. In: Advanced Materials. 2013 ; Vol. 25, No. 36. pp. 5011-5028.
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abstract = "With advances in tissue engineering, the possibility of regenerating injured tissue or failing organs has become a realistic prospect for the first time in medical history. Tissue engineering - the combination of bioactive materials with cells to generate engineered constructs that functionally replace lost and/or damaged tissue - is a major strategy to achieve this goal. One facet of tissue engineering is biofabrication, where three-dimensional tissue-like structures composed of biomaterials and cells in a single manufacturing procedure are generated. Cell-laden hydrogels are commonly used in biofabrication and are termed bioinks. Hydrogels are particularly attractive for biofabrication as they recapitulate several features of the natural extracellular matrix and allow cell encapsulation in a highly hydrated mechanically supportive three-dimensional environment. Additionally, they allow for efficient and homogeneous cell seeding, can provide biologically-relevant chemical and physical signals, and can be formed in various shapes and biomechanical characteristics. However, despite the progress made in modifying hydrogels for enhanced bioactivation, cell survival and tissue formation, little attention has so far been paid to optimize hydrogels for the physico-chemical demands of the biofabrication process. The resulting lack of hydrogel bioinks have been identified as one major hurdle for a more rapid progress of the field. In this review we summarize and focus on the deposition process, the parameters and demands of hydrogels in biofabrication, with special attention to robotic dispensing as an approach that generates constructs of clinically relevant dimensions. We aim to highlight this current lack of effectual hydrogels within biofabrication and initiate new ideas and developments in the design and tailoring of hydrogels. The successful development of a printable hydrogel that supports cell adhesion, migration, and differentiation will significantly advance this exciting and promising approach for tissue engineering.",
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Malda, J, Visser, J, Melchels, FP, Juengst, T, Hennink, WE, Dhert, WJA, Groll, J & Hutmacher, DW 2013, '25th Anniversary Article: Engineering Hydrogels for Biofabrication', Advanced Materials, vol. 25, no. 36, pp. 5011-5028. https://doi.org/10.1002/adma.201302042

25th Anniversary Article : Engineering Hydrogels for Biofabrication. / Malda, Jos; Visser, Jetze; Melchels, Ferry P.; Juengst, Tomasz; Hennink, Wim E.; Dhert, Wouter J. A.; Groll, Juergen; Hutmacher, Dietmar W.

In: Advanced Materials, Vol. 25, No. 36, 25.09.2013, p. 5011-5028.

Research output: Contribution to journalLiterature review

TY - JOUR

T1 - 25th Anniversary Article

T2 - Engineering Hydrogels for Biofabrication

AU - Malda, Jos

AU - Visser, Jetze

AU - Melchels, Ferry P.

AU - Juengst, Tomasz

AU - Hennink, Wim E.

AU - Dhert, Wouter J. A.

AU - Groll, Juergen

AU - Hutmacher, Dietmar W.

PY - 2013/9/25

Y1 - 2013/9/25

N2 - With advances in tissue engineering, the possibility of regenerating injured tissue or failing organs has become a realistic prospect for the first time in medical history. Tissue engineering - the combination of bioactive materials with cells to generate engineered constructs that functionally replace lost and/or damaged tissue - is a major strategy to achieve this goal. One facet of tissue engineering is biofabrication, where three-dimensional tissue-like structures composed of biomaterials and cells in a single manufacturing procedure are generated. Cell-laden hydrogels are commonly used in biofabrication and are termed bioinks. Hydrogels are particularly attractive for biofabrication as they recapitulate several features of the natural extracellular matrix and allow cell encapsulation in a highly hydrated mechanically supportive three-dimensional environment. Additionally, they allow for efficient and homogeneous cell seeding, can provide biologically-relevant chemical and physical signals, and can be formed in various shapes and biomechanical characteristics. However, despite the progress made in modifying hydrogels for enhanced bioactivation, cell survival and tissue formation, little attention has so far been paid to optimize hydrogels for the physico-chemical demands of the biofabrication process. The resulting lack of hydrogel bioinks have been identified as one major hurdle for a more rapid progress of the field. In this review we summarize and focus on the deposition process, the parameters and demands of hydrogels in biofabrication, with special attention to robotic dispensing as an approach that generates constructs of clinically relevant dimensions. We aim to highlight this current lack of effectual hydrogels within biofabrication and initiate new ideas and developments in the design and tailoring of hydrogels. The successful development of a printable hydrogel that supports cell adhesion, migration, and differentiation will significantly advance this exciting and promising approach for tissue engineering.

AB - With advances in tissue engineering, the possibility of regenerating injured tissue or failing organs has become a realistic prospect for the first time in medical history. Tissue engineering - the combination of bioactive materials with cells to generate engineered constructs that functionally replace lost and/or damaged tissue - is a major strategy to achieve this goal. One facet of tissue engineering is biofabrication, where three-dimensional tissue-like structures composed of biomaterials and cells in a single manufacturing procedure are generated. Cell-laden hydrogels are commonly used in biofabrication and are termed bioinks. Hydrogels are particularly attractive for biofabrication as they recapitulate several features of the natural extracellular matrix and allow cell encapsulation in a highly hydrated mechanically supportive three-dimensional environment. Additionally, they allow for efficient and homogeneous cell seeding, can provide biologically-relevant chemical and physical signals, and can be formed in various shapes and biomechanical characteristics. However, despite the progress made in modifying hydrogels for enhanced bioactivation, cell survival and tissue formation, little attention has so far been paid to optimize hydrogels for the physico-chemical demands of the biofabrication process. The resulting lack of hydrogel bioinks have been identified as one major hurdle for a more rapid progress of the field. In this review we summarize and focus on the deposition process, the parameters and demands of hydrogels in biofabrication, with special attention to robotic dispensing as an approach that generates constructs of clinically relevant dimensions. We aim to highlight this current lack of effectual hydrogels within biofabrication and initiate new ideas and developments in the design and tailoring of hydrogels. The successful development of a printable hydrogel that supports cell adhesion, migration, and differentiation will significantly advance this exciting and promising approach for tissue engineering.

KW - bioprinting

KW - biofabrication

KW - additive manufacturing

KW - hydrogel

KW - biomaterials

KW - biopolymers

KW - DOUBLE-NETWORK HYDROGELS

KW - 3-DIMENSIONAL FREEFORM FABRICATION

KW - CELL-LADEN

KW - ENDOTHELIAL-CELLS

KW - CARTILAGE REPAIR

KW - CROSS-LINKING

KW - BIOMEDICAL APPLICATIONS

KW - EXTRACELLULAR MATRICES

KW - PRINTING APPLICATIONS

KW - TRIBLOCK COPOLYMERS

U2 - 10.1002/adma.201302042

DO - 10.1002/adma.201302042

M3 - Literature review

VL - 25

SP - 5011

EP - 5028

JO - Advanced Materials

JF - Advanced Materials

SN - 0935-9648

IS - 36

ER -

Malda J, Visser J, Melchels FP, Juengst T, Hennink WE, Dhert WJA et al. 25th Anniversary Article: Engineering Hydrogels for Biofabrication. Advanced Materials. 2013 Sep 25;25(36):5011-5028. https://doi.org/10.1002/adma.201302042