3D microrheology using optical tweezers with integrated multiplane microscopy

Activity: Talk or presentationInvited talk

Description

I will discuss a 3D microrheology system that combines Optical Tweezers with Integrated Multiplane Microscopy. 3D tracking of an optically trapped bead, is achieved with ~ 20 nm accuracy along the optical axis. We have developed a straightforward image sharpness spatial calibration method using images of a trapped particle that have been captured simultaneously from multiple planes. The viscosity of water has been measured simultaneously in three dimensions, showing the effectiveness of the technique. I will also discuss some future prospects for the system to measure the mechanical properties of biological material.
Period29 Sep 2021
Event titleInternational Society of Optics and Photonics (SPIE) Frontiers in Biophotonics and Imaging
Event typeConference
LocationGlasgow
Degree of RecognitionNational