DescriptionI will discuss a 3D microrheology system that combines Optical Tweezers with Integrated Multiplane Microscopy. 3D tracking of an optically trapped bead, is achieved with ~ 20 nm accuracy along the optical axis. We have developed a straightforward image sharpness spatial calibration method using images of a trapped particle that have been captured simultaneously from multiple planes. The viscosity of water has been measured simultaneously in three dimensions, showing the effectiveness of the technique. I will also discuss some future prospects for the system to measure the mechanical properties of biological material.
|Period||29 Sep 2021|
|Event title||International Society of Optics and Photonics (SPIE) Frontiers in Biophotonics and Imaging|
|Degree of Recognition||National|
Documents & Links
Research output: Contribution to journal › Article › peer-review