3D microrheology using optical tweezers with integrated multiplane microscopy

Description

I will discuss a 3D microrheology system that combines Optical Tweezers with Integrated Multiplane Microscopy. 3D tracking of an optically trapped bead, is achieved with ~ 20 nm accuracy along the optical axis. We have developed a straightforward image sharpness spatial calibration method using images of a trapped particle that have been captured simultaneously from multiple planes. The viscosity of water has been measured simultaneously in three dimensions, showing the effectiveness of the technique. I will also discuss some future prospects for the system to measure the mechanical properties of biological material.

Period	29 Sept 2021
Event title	International Society of Optics and Photonics (SPIE) Frontiers in Biophotonics and Imaging
Event type	Conference
Location	GlasgowShow on map
Degree of Recognition	National